In vitro DNA/RNA Adductomics to Confirm DNA Damage Caused by Benzo[a]pyrene in the Hep G2 Cell Line
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Evaluation of DNA damage in a Her2+ cell line induced by an Auger-emitting immunoconjugate
Introduction: Auger electron based radioimmunotherapy (RIT) using 111In-DOTA-trastuzumab (111In-DOTA-antiHer2) feasibility was studied in vitro on a HER2/neu positive cell line, SkBr3. Methods:111In-DOTA-antiHer2 was prepared according to the optimized conditions followed by quality control tests including radioch...
متن کاملHep G2 is a hepatoblastoma-derived cell line.
[1] Brabletz T, Jung A, Spaderna S, Hlubek F, Kirchner T. Migrating cancer stem cells—an integrated concept of malignant tumor progression. Nat Rev Cancer 2005;5:744-9. [2] Carr I, Levy M, Watson P. The invasive edge: invasion in colorectal cancer. Clin Exp Metastasis 1986;4:129-39. [3] Gabbert H, Wagner R, Moll R, Gerharz CD. Tumor dedifferentiation: an important step in tumor invasion. Clin E...
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Confluent monolayers of the human hepatoblastoma-derived cell line, Hep G2, were incubated in serum-free medium. Conditioned medium was ultracentrifugally separated into d < 1.063 g/ml and d 1.063-1.20 g/ml fractions since very little VLDL was observed. The d < 1.063 g/ml fraction was examined by electron microscopy; it contained particles of 24.5 * 2.3 nm diameter, similar in size to plasma LD...
متن کاملDNA Adductomics
Systems toxicology is a broad-based approach to describe many of the toxicological features that occur within a living system under stress or subjected to exogenous or endogenous exposures. The ultimate goal is to capture an overview of all exposures and the ensuing biological responses of the body. The term exposome has been employed to refer to the totality of all exposures, and systems toxic...
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Receptor-mediated endocytosis of tissue-type plasminogen activator (t-PA) was characterized with the human hepatoma cell line Hep G2. At 4 degrees C binding of 125I-t-PA to Hep G2 cells is rapid, specific, saturable, and reflective of a homogeneous population of 76,000 high-affinity surface sites per cell (Kd = 3.7 nM). The kinetics of 125I-t-PA binding to its receptor are characterized by rate...
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ژورنال
عنوان ژورنال: Frontiers in Chemistry
سال: 2019
ISSN: 2296-2646
DOI: 10.3389/fchem.2019.00491